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Definition

To set up people or things so as to be ready for their cues (such as backstage).

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How People Actually Use It

When a man uses a shot glass full of vasoline that he keeps in his pocket to lubricate his penis quickly before sex.

"I met a girl the other night at the club and we had sex out in my car. Thank god i had my shot glass with me and i could cue up."

Source: Urban Dictionary